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Page IRuf41 <br />I�cmtit No. �1'A,00314Z-G <br />htodilication Datc: Mav I. 2007 <br />S8. ACUTE 1'OXICCI'1' <br />A. 1�.171ucni Charactcrization <br />I. Thc Pcrmiltcc shall conduct a minimum nf thrcc (3) scparatc acutc tosiciq <br />icsts on thc final cftlucnt ai Outlall Y,001 to dctcrminc thc prcscncc and <br />amount of acutc (lethal) to�icit}. <br />2. fhe first aciuc tosicih tcs� shall bc conductcd simult:mrnusl�� with thc first <br />dischargc at Outfall k001 following complction of �he li�i slation, climination <br />of OwGill �1005, and thc rcdirccting of Outfall k005 waters to Outfall t(001. <br />l�hc sccond and ihird acutc toxiciry �csts shall bc conductcd during differcnt <br />dischargc c�cNs, and cach as closc �o critical conJi�ions as possiblc. <br />i. If ihc thrcc cansccutive acutc tosicit� tats result in 100% sun�ival in 100°0 <br />cfllucnt, no additional acutc tosiciiy Icsting �aill be rcyuircd of thc <br />Pcrmittcc to thc c�piratiun datc of thc pennit. <br />4. All acute to�icii�� tests lis�cd bclo�e shall hc conducicd on each samplc <br />takcn tur cfllucn[ charactcrization. A wriltcn rcport ollhc toxicih Icsls <br />cillucnt char.icteriration data shall be submitted to the Uepariment �� ithin <br />sixt� (60) da}'s after the sample date. Ifany of�hc ihree acute to�icit� tesu <br />�mdcr numbcr I in this subsection do no1 resuh in 100';'a sun�ival in 10090 <br />eillucnt. thcn the Pcrmittee shall suhmit a�ariuen report of the acute <br />losici��� tcsls charactcrization to thc Dcparimcnt cach permit c}cic. �fhc <br />Dcpariment must receivc thc rcport �� ithin ninch (901 days follo�� ing the <br />last charactcrization sampling e�ent. <br />Acutc tu�icin� irs�ing shall (ollo�c protucols, monitoring rcyuircnunts, anJ yualitc <br />a,surancc/yuality conVoi proccdures spccificd in ihis scction. A dilu�ion scrics <br />consisting of a minimum of �i�c conccntrations and a control shall bc uscJ to <br />cs�imalc thc conccntr;uion Icthal to 50%of thc organisms (LCsi,). Thc perccnt <br />wrvival in I OO��o l'R�UI'OI SII:III 8IS0 I1C fl'�lOfll'(I. <br />Acute toaici�}• icsts shall bc cunductcd �� ith thc ti�llo�� ing spccics and prutocols: <br />I. �hsiJ shrimp,:l�ru�ricnn��•.ei.c haliiu (uku Alr.vidup.cis hnhiul <br />(�18-hour slatic test, mcthod: EPA R? I/R-0?-01'). <br />Z. Rainbo�e UouL (hrrurinnchtcc nirki.cc (�)(,-h�wr s�a�io-rcnc�v��l �cst. <br />mcthod: f:PA 821/R-02•012). <br />Ii1�11ucni I.inti� �i�r Aculc'foxicil� <br />I'hc Pcrmit�cc will havc an efilucnt limi� I'or acutc tosicity il'any of thc thrcc (31 <br />acwe toxicity ics�s (required under mm�ber I in subsection A) on the f inal etllucnt <br />at OutG�ll N001 resull in ci�hcr: <br />I. 'I�hc mcdian survival of a�q� specics in 1009� cfllucnt is bclow R09o. <br />2. Any one tcst ef any specics e�hibits Icss than GS% survival in 100% efFluent. <br />If an cfllucnt limit for acutc tosicit� is rcyuircd by (I115 SUIISCC[IOII �I3�. Illl' <br />Permiuce shall immediatel�� completc all applicablc requiremenis for routinc <br />m.mitoring :md compliancc �vi�h thc acutc 11T_T limit in subscctions C, 1), and P. <br />