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<br />2 of 11 <br /> <br /> <br />2. Equipment required: <br />a. Nitrile gloves <br />b. Seven (7) two (2)-liter high-density polyethylene (HDPE) sample containers <br />c. Sharpie pens, labels for sample containers <br />d. Chain-of-Custody form <br />e. Pond map showing designated sample sites <br />f. Sludge sampler (8-10 feet long) <br />g. Garmin Chartplotter <br />h. Square D key <br />i. Life jackets <br />j. Paddles <br />k. Barge, bio boat, or dinghy <br />3. Time to complete: 2-4 hours <br /> <br />E. Procedure <br />1. Sample collection occurs tri-annually in the Aeration cells and Recirculation Channel, and <br />annually in the Oxidation Pond, plus an independent collection for VAR testing between 45-60 <br />days prior to a dredging event. <br />2. AC-2 is divided into 14 discrete sampling sections (A-N). Samples are collected from areas A-G <br />on the first sampling week of the quarter, and from sites H-N on the second sampling week of <br />the quarter. The Recirculation Channel is divided into 7 discrete sampling sections (S-U, and W- <br />Z), and all Recirculation quarterly samples can be collected in a single week. The Oxidation Pond <br />is divided into 18 discrete sampling sections (A-R). Samples are collected from areas L-R on the <br />first sampling week of the year, from sites E-K on the second sampling week of the year, and <br />from sites A-D, on the third sampling week of the year. This allows the Biosolids Area Lead to <br />track and trend analyte values in each area over time. <br />3. For VAR testing, collect one core from each of the discrete sampling sections into individual <br />pre-labeled bottles. For AC-2, collect 14 discrete samples, A-N. For the Oxidation Pond, collect 7 <br />discrete samples, L-R. For the Recirculation Channel, collect 7 discrete samples, S-U and W-Z. <br />Deliver to the lab for appropriate compositing and analysis. <br />4. A maximum of seven discrete sections can be sampled on a given day due to lab capacity <br />constraints. <br />5. Samples need to be collected on a Monday or Tuesday due to lab staffing and shipping <br />constraints. <br />6. Gather Equipment and Supplies per Section D. Sampling supplies are stored in the Everett <br />Environmental Lab and in the Operations tool corner in Stage III. If needed, the dinghy is stored <br />on the asphalt pad west of the Primaries. <br />7. Label and date the lab sample containers before collecting the samples. <br />8. Determine which vessel will be needed for sampling. The barge is used for sampling AC-2, the <br />bio boat is used for sampling the Oxidation Pond, and either the barge, if available, the bio <br />boat, or the dinghy can be used for sampling in the Recirculation Channel. <br />a. Prepare the barge for AC-2.